By H. Zuber (auth.), Professor Dr. Maria Elisabeth Michel-Beyerle (eds.)
The workshop on "Antennas and response facilities of Photosynthetic Bac teria" used to be held at Feldafing, Bavaria (F. R. G. )' March 23-25, 1985. This workshop focussed on basic methods with emphasis on constitution, inter activities and dynamics. It assessed structural, spectroscopic and dynamic information that have gathered lately, offering an summary of the mech anism of the purchase, garage and helpful disposal of power in bacterial photosynthesis. This quantity is a checklist of the invited papers awarded on the workshop. the fabric was once equipped into 5 sections: I. Antennas: constitution and effort move II. response facilities: constitution and Interactions III. Electron move: conception and version platforms IV. response Cen,ters: constitution and Dynamics V. version structures on functionality of Antennas and response facilities i need to precise my gratitude to all of the contributors within the paintings store for his or her contributions, and to the authors for the well timed guidance in their manuscripts. i'm indebted to the contributors of the organizing committee, Professors Sighart F. Fischer and Hugo Scheer for his or her most beneficial suggestions and suggestion. The workshop don't have been such a success with out assistance from my secretary, Frau Petra KahlfuB, and my coworkers in its association. I thank Frau KahlfuB relatively additionally for her counsel within the practise of those lawsuits. The workshop used to be prepared less than the auspices of the Technical Uni versity of Munich, the Max-Planck-Society and the college of Munich.
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Oj Fig. 8: Heat denaturation of PC. All spectra were recorded 5min after the sample had reached the temperature indicated. All measurements were done with fresh samples. Other details as in Fig. 5. , ................ ,...... ; " \. •. \ \ ... \ \.... \ so \ ~ \ \ \ \ \ \ \ q\.. \ \ \ \ ... \ ... "\. •. \ \ ... \. ~ '. oj ---~:;;. ~-- 20 60 40 80 ·C t--------------.. . _" ··· · ··· ·· ·· ·· ~ . f •. • •f f ....... ..... .. _-s '--_ _ _ _ _ _ _ _ _ _ _ _ _--1 ••f .... Fig. 9: Difference spectra for the photoreaction of PC in the presence of urea (5M).
JJ ~ '\ ~2 ~2 l Fig. 1: Chromophor arrangement in C-PC crystals from Mastigocladus laminosus. Displayed are the least-squares fit-lines for the conjugated systems of the chromophors. The unit cell contains two trimers, the chromophors of which are marked by black and white arrows, resp •• a) View against the crystallographic c-axis, one monomer is outlined by dots. b) View perpendicular to c-axis along the short diagonal of the ab - rhomb 110. g. with the linker peptides. In this paper we report on measurements of the fluorescence decay of crystals of C-PC prepared identically as those used for the X-ray work .
Small aggregation-induced changes have been discussed in the first part. g. with urea or heat. After a previous study with PC from S. platensis, this method has now been used to investigate the stabilitieS-of the PC and its subunits from M. laminosus. The normalized absorption changes upon urea- denaturation are shown in fig. 6 (see legend for details). The gross changes are similar for the integral pigment (trimer) and its subunits. Small differences in the final (= 8M urea) absorptions are indicative of slightly different conformations of native chromophores, because the chromophores have the same molecular structure and are expected to have identical spectra in the denatured states.